Transposon Mutagenesis and Complementation of the Fructokinase Gene in Rhizobium leguminosamm biovar trifolii
نویسندگان
چکیده
منابع مشابه
The Rhizobium Ieguminosarum biovar viciae nod0 gene can enable a nod€ mutant of Rhizobium leguminosarum biovar trifolii to nodulate vetch
Sciences, University of East Anglia, Norwich NR4 7TJ, UK 2 School of Biological Analysis of the nodulation characteristics of transposon-induced mutants of Rhizobium leguminosarum bv. viciae revealed that nodO and the closely-linked rhi genes contribute t o nodulation of peas (Pisum sativum) and the vetch Vicia hirsuta. Although mutation of nodO alone had no significant effect on nodulation of ...
متن کاملDirected transposon Tn5 mutagenesis and complementation analysis of Rhizobium meliloti symbiotic nitrogen fixation genes.
An 18 kb region adjacent to and surrounding the genes for nitrogenase (nif) was cloned from the genome of the symbiotic nitrogen-fixing species Rhizobium meliloti. A total of 31 Tn5 insertions in the nif region were constructed and assayed for their effect on symbiotic nitrogen fixation (Fix phenotype). Fix- insertions were found in two clusters, one 6.3 kb region not containing essential symbi...
متن کاملThe dynamic response of cellular carbohydrates to a NaCl shock in Rhizobium leguminosarum biovar trifolii TA-
The dynamic response of cellular carbohydrates to a NaCl shock in Rhizobium leguminosarum biovar trifolii TA1 (0.25 M-NaCI) and Rhizobium meliloti SU-47 (0.4 M-NaCl) grown in NaC1-free medium was investigated in nongrowing cell cultures and in cell suspensions, using in vivo NMR. After transferring cells grown in a NaC1-free medium to a glutamic-acid-free medium containing mannitol and NaCI, bo...
متن کاملEnvironmental Factors Influencing Numbers of Rhizobium leguminosarum biovar trifolii and Its Bacteriophages in Two Field Soils.
Fluctuations in numbers of Rhizobium leguminosarum biovar trifolii and its bacteriophages in two fields with different soil types were followed during a 17-month period in 1981 and 1982. Mean levels of both phage and rhizobia varied significantly (P < 0.05) on different occasions, with rhizobial levels varying from 1.6 x 10 to 2.0 x 10 cell per g of soil and phage from 0 to 1.7 x 10 PFU/g of so...
متن کاملCell-associated pectinolytic and cellulolytic enzymes in Rhizobium leguminosarum biovar trifolii.
The involvement of Rhizobium enzymes that degrade plant cell wall polymers has long been an unresolved question about the infection process in root nodule symbiosis. Here we report the production of enzymes from Rhizobium leguminosarum bv. trifolii that degrade carboxymethyl cellulose and polypectate model substrates with sensitive methods that reliably detect the enzyme activities: a double-la...
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ژورنال
عنوان ژورنال: Microbiology
سال: 1989
ISSN: 1350-0872,1465-2080
DOI: 10.1099/00221287-135-8-2329